Jaya Krishnan U

8 Questions 5 Answers 0 Followers

Questions related from Jaya Krishnan U

Is there any direct HPLC method for quantification of PHB,PHV using C-18 Reverse Phase column?

* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...

01 January 2020 2,887 2 View

How to make Standard curve for Crotonic acid assay and use it for estimation of Polyhydroxyalcanote from activated sludge?

Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...

03 March 2019 8,548 1 View

How to determine polyhydroxybutyrate and polyhydroxyvalerate quantitatively through crotonic acid assay?

I found a procedure for quantitative analysis of PHB and PHV using crotonic acid assay at 235nm and 285nm respectively. But scanning the standard I was unable to get a peak at 285nm even though...

02 February 2018 1,196 0 View

GC is producing no peaks or sometime abnormal peaks of VFA in water. I used to get good quality peaks initially. What could be possible reason?

The Thermo Scientific Trace GC Ultra fitted with DBWAXTER produced good quality peaks of VFA (in water ) initially after installation. But when I tried analysis after 2 months, ended up in...

06 June 2017 2,158 2 View

What kind of filter paper is ideal for volatile suspended solid analysis? and pore size 0.45-2 micron?

Glass microfiber filter is chemically stable, stable at 500ºC, mechanical strength etc.,. wouldn't that make glass microfiber unsuitable for VSS. While Ashless filter paper leaves no ash after...

10 October 2016 521 0 View

Can anyone give me an idea regarding use of aqueous sample in nonpolar/polar columns in GC for fatty acid analysis?

Will there be any problem with the column if I run acetic acid dissolved in water? The query is with respect to the nonpolar and polar column.

08 August 2016 9,435 8 View

Sensitivity of Montegomery Method for Acetic acid estimation for Concentrations below 100ppm?

I was able to construct a standard curve for acetic acid using above-mentioned method for 200-1000ppm. But I failed many a time for the same for concentrations 20-100ppm. After the entire reaction...

08 August 2016 4,614 1 View

Is it possibile to maintain an anaerobic mix culture using defined media?

An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...

06 June 2016 3,629 5 View