Is there any comparison between the number or frequency of existing G4 qudruplexes in mycobacterium and E.coli?

10 October 2018 0 5K Report

I wanted to know how to analyze the existence of qudruplexes DNA in Mycobacterium can be compared to E.coli

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More Balaji M's questions See All

Inspiralis customers can you please answer this?

Gyrase supplied by inspiralis says 1U but what is the concentration of the enzyme that they provide. How to do inhibition studies based on what concentration. Plz shed some light over this

02 March 2018 7,770 2 View

How to make the A2B2 form of gyrase?

Recently I am involved in studying gyrase protein. To make a functional holoenzyme, it should be in A2B2 form. So, I trying to form this complex but need an expertise from you to achieve it. Thank...

02 March 2018 7,508 5 View

Can I get a protocol to isolate supercoiled plasmid DNA?

Acid phenol extraction seems to be feasible in my lab (A/c to the facilities available). I could not find out the protocol to fish only the supercoiled form of plasmid. Please shed some...

31 December 2017 8,890 4 View

How should i understand this; the microbes that produce antibiotics are specific against a group/competitor for same substrate?

or is it a chance that these intermediates are just chucked and by chance these are active against particular organisms. Can someone enlight me in this case with some publications. Thank you

02 March 2016 333 3 View

Can someone suggest me what kind of a compound it may be based on the Uv spectrum?

Somebody suggest me the database please?

02 March 2016 5,515 11 View

Can someone clarify my TLC Results for me?

Here I show a compound seperated through TLC and got a single spot. My hitch is under longer UV I see a green fluroscent centre and a blue periphery. So does this blue reveal any scattering effect...

08 September 2014 10,109 33 View

Can someone help me to describe Streptomyces sp?

Describing a novel species involves justification like 16S r RNA sequencing, FAME analysis etc. Is DNA _DNA hybridization essential if the FAME suggests 87% similarity with the available strains...

07 August 2014 4,742 3 View

Can I get suggestions for determining a molecule - say it is antibiotics - what are all the methods and validations?

I got some active principle say it as an antibiotic. In order to know the exact molecule what are all the validations and methods I should employ to find its structure. Kindly brief each and...

01 February 2014 6,338 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View