Inspiralis customers can you please answer this?

Gyrase supplied by inspiralis says 1U but what is the concentration of the enzyme that they provide. How to do inhibition studies based on what concentration. Plz shed some light over this

02 March 2018 7,680 2 View

How to make the A2B2 form of gyrase?

Recently I am involved in studying gyrase protein. To make a functional holoenzyme, it should be in A2B2 form. So, I trying to form this complex but need an expertise from you to achieve it. Thank...

02 March 2018 7,429 5 View

Can I get a protocol to isolate supercoiled plasmid DNA?

Acid phenol extraction seems to be feasible in my lab (A/c to the facilities available). I could not find out the protocol to fish only the supercoiled form of plasmid. Please shed some...

31 December 2017 8,795 4 View

Can someone suggest me what kind of a compound it may be based on the Uv spectrum?

Somebody suggest me the database please?

02 March 2016 5,418 11 View

How should i understand this; the microbes that produce antibiotics are specific against a group/competitor for same substrate?

or is it a chance that these intermediates are just chucked and by chance these are active against particular organisms. Can someone enlight me in this case with some publications. Thank you

02 March 2016 247 3 View

Can someone clarify my TLC Results for me?

08 September 2014 10,005 33 View

Can someone help me to describe Streptomyces sp?

Describing a novel species involves justification like 16S r RNA sequencing, FAME analysis etc. Is DNA _DNA hybridization essential if the FAME suggests 87% similarity with the available strains...

07 August 2014 4,656 3 View

Can I get suggestions for determining a molecule - say it is antibiotics - what are all the methods and validations?

I got some active principle say it as an antibiotic. In order to know the exact molecule what are all the validations and methods I should employ to find its structure. Kindly brief each and...

01 February 2014 6,254 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View