I got some active principle say it as an antibiotic. In order to know the exact molecule what are all the validations and methods I should employ to find its structure. Kindly brief each and possible references. Though FTIR , NMR and MALDI can help I wanted to know any thing else is still available new?
Ameriga Lazzarini
Before going to NMR you need MW, UV spectrum, mechanism of action, a panel of antibacterial activities also comprising strains resitent to known classes of antibiotics, to check in literature for already known compounds ( about 20,000 are the microbial products).
Also stability of the molecule at 3 pH is useful.
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