Is there any comparison between the number or frequency of existing G4 qudruplexes in mycobacterium and E.coli?

I wanted to know how to analyze the existence of qudruplexes DNA in Mycobacterium can be compared to E.coli

09 October 2018 4,437 0 View

How to make the A2B2 form of gyrase?

Recently I am involved in studying gyrase protein. To make a functional holoenzyme, it should be in A2B2 form. So, I trying to form this complex but need an expertise from you to achieve it. Thank...

02 March 2018 7,429 5 View

Can I get a protocol to isolate supercoiled plasmid DNA?

Acid phenol extraction seems to be feasible in my lab (A/c to the facilities available). I could not find out the protocol to fish only the supercoiled form of plasmid. Please shed some...

31 December 2017 8,795 4 View

Can someone suggest me what kind of a compound it may be based on the Uv spectrum?

Somebody suggest me the database please?

02 March 2016 5,418 11 View

How should i understand this; the microbes that produce antibiotics are specific against a group/competitor for same substrate?

or is it a chance that these intermediates are just chucked and by chance these are active against particular organisms. Can someone enlight me in this case with some publications. Thank you

02 March 2016 247 3 View

Can someone clarify my TLC Results for me?

08 September 2014 10,005 33 View

Can someone help me to describe Streptomyces sp?

Describing a novel species involves justification like 16S r RNA sequencing, FAME analysis etc. Is DNA _DNA hybridization essential if the FAME suggests 87% similarity with the available strains...

07 August 2014 4,656 3 View

Can I get suggestions for determining a molecule - say it is antibiotics - what are all the methods and validations?

I got some active principle say it as an antibiotic. In order to know the exact molecule what are all the validations and methods I should employ to find its structure. Kindly brief each and...

01 February 2014 6,254 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Person-Codes for questionnaires in longitudinal studies: Is there an easy solution for better matching-rates?

Hi, I have a problem regarding matching of longitudinal data in my field of research (university students). We have a Person-Code for matching data measured in different time points, but 30%+ of...

17 February 2021 3,075 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

What are the latest topics in strategic management area, esp those related to Quantum managment?

I am trying to involve science , in particular Physics, into Strategic Management studies for my master thesis . There are a number of papers discussing this topic but in such a fast changing...

13 February 2021 891 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View