As it is known, an specific microRNA can have multiple mRNA targets. If an microRNA fully base-pairs with its target mRNA, the mRNA will be degraded. In the case of incomplete base pairing different other mechanisms are applied for translation suppression. I don't know if we could predict an mRNA or gene as the most affected mRNA/gene target for an specific microRNA. Is there any software or application that via exploiting the complete or incomplete matching of microRNA-mRNA pairs or hydrogen bond interactions (or by some other means) could be able to identify the most important target of an microRNA (regardless of the specific cell/disease contexts in which our study is being conducted or relative abundance of mRNA transcripts), as what is observed in the case of protein docking?
Hi Mohsen,
you should try to look at the Mir Baes (http://www.mirbase.org) where you'll find miRNA and targets.
fred
Hello!
Have a look here:
www.microrna.org
www.targetscan.org
Also the company exiqon has an libarary with a lot of publication to nearly all miRs.
Predictive algorithm:
http://www.targetscan.org/vert_71/
https://cm.jefferson.edu/rna22/
http://zmf.umm.uni-heidelberg.de/apps/zmf/mirwalk2/
If you know the cell lines, you may want to find out from actual CLIP-seq data for the core microRNA binding protein AGOs 1-4: http://lulab.life.tsinghua.edu.cn/clipdb/
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