Is there any alternative for Prioritizing SNP according to their probable functional impact using fastSNP?

More Moustafa Nouh Elemeery's questions See All

Can biofloc system be applied in earthen ponds?

Dear colleagues Can I apply biofloc system in earthen ponds?

17 May 2024 2,685 0 View

High dsDNA concentration on Qubit but horrible, degraded gel electrophoresis?

Hello everyone! I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's...

15 December 2023 4,751 1 View

Hello every one... Could you guid me to interpertate dielectric spectrogram and cole cole plot especially for the main variables or parameters ?

Dielectric permitivity spectrogram interpertation and cole cole plot especially for biological material

09 March 2023 5,691 0 View

Is there a commercially available kit for removing mouse proteins from serum or tissues?

I am attempting to detect a number of proteins expressed by a bacterium during infection in a mouse model. However, the presence of mouse proteins in the serum and tissues is hindering the results...

02 February 2023 2,629 1 View

Why is there in our time a decline in education and learning?

Has the development of technology led the human mind, especially the educated, to a lack of absorption and difficulty in learning despite the availability of technological means, unlike the...

27 October 2022 7,633 14 View

Surface plasmon resonance sensogram dotted and solid lines?

Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks

19 May 2022 1,258 3 View

How can i dissolve Manganese (II) chloride in water?

I need to dissolve it in water for the preparation of SEM-TB media. I prepared 1M stock solution but it is cloudy not clear. I can not use it in the media preparation as it has to be clean.So what...

01 December 2021 3,545 5 View

Is there anyone who can give me some studies and statistics for brain drain from North Africa to Europe and North America?

01 November 2021 7,256 3 View

How to get my final stock solution??

I have a drug that comes in 10mg, how much would I add to obtain a final stock concentration of 100mM? The MW is 579.9and the drug is soluble in H2O at 52mg/ml. When I did the calculation I...

18 October 2021 2,745 3 View

Are there free QSAR software for Genotoxicity prediction that support expert-based and statistical-based models?

International Conference of Harmonization (ICH) guideline M7 recommends using two complementary QSAR models, expert-based and statistical-based models, for Genotoxicity prediction. I'd be thankful...

12 August 2021 4,357 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Are there any alternatives to PhenoScanner?

I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...

23 July 2024 5,703 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Can someone please explain to me what power law statistics mean in single molecule fluorescence blinking ?

I am a beginner in this field. While I am reading papers and the understanding is getting better. However can someone explain in easy and simple words. Really thankful !

08 July 2024 8,337 5 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

How would I calculated ddPCR spiked nucleic acid recovery calculations?

Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...

02 July 2024 6,011 2 View