Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How many samples/measurements do I need for certain a sensitivity & specificity?

I'm taking some measurements using Raman spectroscopy of healthy pig eye samples using a new excitation wavelength and my supervisor said to choose a sample size to allow for spec/sens of 85%....

01 November 2020 8,611 6 View

Inconsistent HPLC results grouped into two value groups?

Dear all, I'm running a reversed-phase HPLC protocol for Irinotecan using standards. I'm getting for each standard very inconsistent peak areas. The peak areas are grouped however in two groups...

22 June 2020 8,347 3 View

What statistical analysis will I need?

I have taken data from two groups, different participants (control and intervention group) over 3 time points. What analysis on SPSS do I need to do to see if there is a significant difference...

17 February 2020 9,363 5 View

Why is my media turning black?

I have a cell line that causes DMEM to turn a dark purple/black colour when the cells are 100% confluent. However, this only happens when the cells are stably transfected with a specific plasmid,...

17 March 2019 3,621 4 View

Hi! Does anybody know if there is any open access dataset about liver cancer gene expression?

I want to use such a dataset for my research.

20 January 2019 5,585 3 View

Does anybody know of a p53 antibody that works well in Western blot for mouse protein?

Hello, I have consistently had problems with a variety of antibodies for p53 in Western blots (bands at incorrect heights / unspecific bands). I am trying to detect the level of p53 protein from...

14 February 2018 7,197 10 View

SPSS Statistic Analyis; ANOVA or Multiple Regression?

Basically I want to find out public attitudes towards punishment of sexual offenders. I have two scales to measure general attitudes, 6 vignettes which takes into account how the type of offence,...

03 July 2017 9,790 4 View

We need help from a psychologist to edit a paper-we offer co-authorship

We have written a paper entitled: "Can cosmetics advertisements be indicators of different perception of beauty amongst Countries?" We are looking for a psychologist to edit the paper in order for...

04 May 2017 5,880 2 View

Lipid extraction for samples stored in ethanol?

Hello, I'm hoping to extract lipids from some shark muscle samples for some fatty acid analyses. All the literature I can find is based on frozen samples, but mine are stored in ethanol - will...

02 March 2016 9,921 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View