How do I normalize on three reference genes with Pfaffl Method in RT qPCR?

Hi, I have a question on RT qPCR: until now I used Livak method to analyze my qPCR data. I normally use 8 different reference genes and determine the three most stable ones. Then I use the...

10 October 2018 3,719 2 View

Peroxide value of seed oil: Sometimes the consumption of titration solution of the blank value is higher than of the oil sample. Why?

All solutions were prepared fresh (Wheeler method) and seeds were freshly pressed for the oil

13 May 2018 9,980 2 View

How to get rid of persistent cell culture contamination?

We are having a problem with contamination in our cell culture. Since a couple of months we are getting frequent but isolated contaminations with bacteria (we think it might be E. coli). We...

21 January 2018 2,650 3 View

Any advice on how to derive clones from hIPSCs, stably transfected with inducible overexpression constructs (problem: spontaneous differentiation)?

I need to derive human IPSC clones from a bulk population that was stably transfected with Dox-inducible (TetOn) overexpression constructs, using the piggybac / transposase system. I did the...

31 October 2017 1,312 2 View

What process variant of shear cutting is applicable for shearing of fibre reinforced plastics?

For some application is it necessary to achieve a high qualitiy of the cutting surface. To what extend it will be possible to transfer the effects from shearing of sheet metal materials to...

14 May 2017 2,847 3 View

Coral/Mangrove Conservation Work in India?

Looking for marine scientific contacts all around India, especially people working in Coral and Mangrove Conservation? Who knows where institutes are located, where projects can be joined and...

22 November 2016 9,173 6 View

Does anyone know how storing fresh soil samples in a vacuum sealed bag affects soil microbial activity?

I need to store fresh soil samples for several weeks and want to avoid lysing microbial cells (so I cannot sterilize the soil), but at the same time minimise microbial activity.

08 May 2016 194 6 View

How can one measure sex hormones in mice?

22 October 2015 4,236 5 View

What are the ethical implications of cognitive robots?

As we strive to create embodied artificial intelligence with increasingly greater capabilities, do we have a moral responsibility to set ourselves an acceptable limit to the level of intelligence...

13 September 2012 3,127 16 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View