I want to isolate the microbiom from the gut of the larvae but I have problems with the dissection. I tried to use needles and a scalpel from a dissection set for biologists but I had problems to open the larvae and to find the gut. Is there any trick?
The best way of dissect the caterpillar by pin aping system. At make a hole in paraffin base tray according the size of larvae and pin up the first and last part of the body and also opening of the middle part.
To dissect any larvae, you will need fine forceps and hold the larva and put the forceps in the mouth parts of the larvae and pull the parts of the mouth outwards and you will see the digestive system connected to the parts of the mouth.
You have to put them in water. It depends also on what type of investigation you Re after.which instar you are also using.
How do you differentiate larvae of other insect order from Lepidotera?
Larvae of Lepidoptera they have well developed head capsule, 3 pairs of thoracic leg and 3-5 abdominal leg; Duster headless and legless; Coleopyeta well develped.head capsule and only three pairs of thoracic leg; Hymenopteta well developed head capsule. Three pairs of thoracic legs and greater than five pairs of abdominal leg.
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