Normally I would extract the DNA and then sequence it, but this is just a small experiment, which need do be done fast. I looked up selective media for Pseudomonas spp. and found a few: CFC agar, NAA agar with trimethoprim, Pseudomonas isolation agar and ChromAgar. As King's B medium only detects fluorescent Pseudomonas, I assumed it would not be suitable for my experiment. Also, do you know if I need to verify the isolated Pseudomonas with additional biochemical tests? oxidase and catalase reaction or if a Pseudomonas specific PCR might be appropriate in this case?
what would you suggest? use non selective and do all the biochem. tests?
Assume you want before and after data in terms of numbers. Your target Pseudomonas spp. per se? pseudomonads?- neither is readily addressed in culture from environmental source.
Think you should start by culturing the target soil with some of these media. If you can get a +/- steady count - you could generate data showing effect in that context. No way you could do phenotypic ID of each colony so your metric would be counts recovered with medium X as surrogate. You can;t claim validation of recovery or exclusively "Pseudomonas spp." - but you can claim de facto impact.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases