I ran a gel electrophoresis with a 1.5% agarose gel stained with 0.5 µg Ethidium Bromide per mL of agarose. When looking at the gel under fluorescence, one half of it remained dark and did not appear to be stained. At first I though it was due to insufficient mixing of EtBr and agarose, but then there would not be a clean cut-off between stained and not-stained parts.
Any suggestions why this happened? Thank you.
Ajay Saini
Hello,
Do EtBr staining after running the gel. It will give a uniform staining and background
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Katie A S Burnette
You can also try adding EtBr to the gel buffer. Put in a few microliters in the end of the tank with the positive electrode and give it a swirl to mix.
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