I have seen a method for calculating the virion count in phage based on a sample's A269 and A320 values.

http://abdesignlabs.com/technical-resources/bacteriophage-spectrophotometry/

This is intended for use in filamentous phage where the protein to DNA ratio is different from in Lambda phage. I am unsure how to modify this calculation for use with Lambda phage.

Any ideas to avoid slow plaque assays would be greatly appreciated!

Thanks

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