No title

09 October 2014 5,593 1 View

Prediction of half life for peptides and proteins: is there a website to assess the half life?

What website could assess the half life of peptides and proteins?

02 March 2014 8,901 1 View

Could someone suggest a website which shows how to calculate the electronic properties of unnatural amino acids?

02 March 2014 3,704 1 View

Is there a website to check if the signaling of GPCR is impacted after mutations?

I would like to test how multiple mutations impact the signaling of GPCR.

02 March 2014 4,512 5 View

Any example of peptides or proteins mutations with regards to Half life?

I would like some learning data sets (WT + a lot of mutants) in relation to half life.

02 March 2014 5,158 0 View

Aggregation of protein and peptides: Is there any example of WT + a lot of mutations in relation to aggregation?

I would like some learning data sets (WT + mutants and aggregation values for each) to assess the robustness of an algorithm.

02 March 2014 9,000 1 View

Is there a website to assess the thermostability of peptides and proteins?

What is the most relevant website to predict the thermostability of peptides?

02 March 2014 7,970 3 View

What are the amino acids residues involved at the active site of Malate Thiokinase?

Malate thiokinase active site residues

11 December 2013 4,061 4 View

What it the best algorithm to predict half life of a peptide or a protein?

What are the best mutations to extend half life of a peptide or a protein?

11 December 2013 9,465 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View