I would check papers in JBC by Hersh, J, et all. Succinate COA synthase uses histidine to make a high energy histdinyl phosphate as an intermediate. Most enzymes that do this types of reaction use histidine for this but it doesn't mean they will use it.
Many thanks Marcia for this information. Do you have any other information about the catalytic pocket of this malate thiokinase or similar enzyme such as Succinyl CoA synthase? I would like to find hot spots in order to go through relevant mutations. Many thanks again. Best.
No I don't know much about these enzymes. The JBC papers can be downloaded online. I would start with them.
I did a quick search in the main enzyme reaction databases (BRENDA, KEGG, CSA, MACiE, SFLD, EzCatDB) and I found some literature references that may have relevant information in BRENDA: http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.9 Good luck with your search.
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