I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which has bound to the antibody. Currently, I am able to determine the binding of the DNA to the Ab by visualizing a band shift following coomassie staining on a native PAGE gel. I have also tried spraying the gel with SyBr Gold prior to fixing the gel for coomassie staining in the hopes of doing an overlay, but this hasn't worked! I know that there is a portion of DNA bound to my Ab as I am able to pull the Ab out of solution and when incubating this conjugate with a restriction enzyme and running this on a PAGE gel I can see the DNA. I am wondering if anybody knows of a way that will allow me to visualize the DNA bound to the Ab without having to do the digest as I would like to run downstream experiments with the conjugate?