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It seems only mRNA or protein was used for cas9 injection in zebrafish. Is there anyone try plasmid cas9 DNA for zebrafish? what is the efficiency? Any good promoter for fish embryo? Is Nos1 good?
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For many years, I have tried occasionally to make competent cell from E coli that was revived from competent cell. It never worked well, normally got super low efficiency, even I tried streaking...
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