After centrifugation, I pass the suspension through the syringe filters, but the bacteria still grows in the culture medium.. How can I completely remove it from the culture? (Autoclaving and tyndallization should not be carried out).
Have you considered bactericidal agents? If you need to neutralize the bactericidal agents afterward, then here are some ideas: https://ethidelabs.com/neutralization-of-antimicrobial-activity/
It is difficult to completely remove all bacteria from a liquid medium after centrifugation, as some bacteria may remain in the pellet or adhere to surfaces even after washing or resuspension steps. However, there are several methods that can be used to reduce bacterial contamination and improve the purity of the sample:
Overall, it is important to carefully consider the specific needs and limitations of the sample and the downstream applications when choosing a method to remove bacteria from a liquid medium. A combination of methods may be necessary to achieve the desired level of purity.
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