Is it possible to introduce a 2D axis-symmetric model in COMSOL Multiphysics to solve electromagnetic waves?

Hello dear researchers. I want to solve a cylindrical waveguide, lick an ordinary standard optical fiber. I am familiar enough with the 2D-axis-symmetric model, but as I knew from the equations...

11 January 2021 6,663 6 View

How can I design a beam splitter?

I would like to make a beam splitter by a glass for a specific angle. First I calculated the thickness of the partially reflecting layer by using of the transfer matrix then I coated this layer by...

25 September 2020 7,283 1 View

Effect of anesthetics drugs on coronavirus patients?

What is the reason for the ineffective or insignificant effect of Neuromuscular relaxants such as Atracurium and anesthetics such as Ketamine on patients with Coronavirus hospitalized in the...

22 September 2020 5,734 4 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

What kind of amides does the tendon consist of?

In visible reflectance spectroscopy performed on a bovine tendon, peaks were observed corresponding to tendon amides. But I do not know these peaks are related to what kind of amides.

16 August 2020 4,411 3 View

How to calculate the Reconnaissance Drought Index (RDI) in DrinC software?

30 April 2020 906 2 View

Where should I register my systematic review and meta-analysis?

Dear colleagues, Is it alright to register a systematic review and meta-analysis (focusing on human health studies) with the International Platform of Registered Systematic Review and...

25 March 2020 5,382 9 View

What are the crystals formed in the phosphate buffer solution at alkaline condition?

There are some crystals formed on the surface of the glass-ceramic substrate (mainly composed of Al, Si, Ca, O) after being treated with PBS (pH> ~10). I know that buffer salt crystalization is...

16 March 2020 4,385 3 View

How can I find data set for fake news?

Hi, Everyone. I am a PhD student and I need some data about fake news from twitter or any other platform that has available data. Can you please let me know how I can find data?

26 January 2020 6,415 12 View

Has any one experience with woun healing assay in a549 cell line?

I am going to study migration in a549 cell line after a treatment but the a549 cells which I am working with show no migration into the space created by a yellow tip even in normal condition (with...

13 August 2019 1,089 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View