Best primers for 16s rRNA sequencing?

Habibeh Hajian Maleki @Habibeh-Hajian-Maleki

07 November 2023 1 5K Report

What are the best primers for 16S rRNA sequencing for identification of plant-pathogenic bacteria in pure cultures? Which primers are the most common?

Ayyaz Khan

27F and 1492R

Badges
Science topic

Similar topics
Analytical Chemistry
Column

More Habibeh Hajian Maleki's questions See All

How can rural business building help to improve rural economy?

15 June 2024 3,462 3 View

How much is the lift coefficients for the shear gradient force and the wall interaction force of NACA0030?

I need lift coefficients of NACA 0030 for simulate DLD microfluidic behavior in cell separation.

10 March 2024 9,796 0 View

Microplastics removal from wastewater?

Hi. I need to remove all microplastics of a wastewater sample without changing any physicochemical properties of the sample. Can filtration by Whatman filters (for example Whatman 589/3 with...

08 March 2024 8,722 3 View

What does both MacConkey and eosin methylene blue agar select for?

They are both used to differentiate gram negative organisms from gram positives. So what is the difference? Which one would be more beneficial?

19 February 2024 5,094 2 View

How do you differentiate Pseudomonas sp., Ralstonia solanacearum and Pectobacterium carotovorum?

How Pseudomonas sp., Pectobacterium carotovorum and Ralstonia solanacearum strains isolated from the same host could be differentiated based on non-molecular methods (biochemical tests, culture...

12 February 2024 4,718 4 View

Is it possible to measure the amount of thermogenesis (turning white fat into brown) using its markers in the blood?

Is it possible to measure the amount of thermogenesis (turning white fat into brown) using its markers LIKE UCP-1 in the blood(PLASMA)?

29 November 2023 7,676 1 View

What type of strong adsorbent based on waste materials do you recommend for urea and water absorption?

Presently, bio-absorbents have been widely studied by researchers. We plan to select a strong adsorbent based on waste materials to absorb urea and water. Therefore, I would appreciate the...

19 November 2023 3,857 5 View

Hello . To understanding the style of three-study articles, I am looking for some examples of them in the field of psychology. can you help me?

I am a student of educational psychology

18 November 2023 2,583 5 View

How can I simulate DLD microfluidics in comsol?

How can I simulate laminar flow and particle tracing for DLD microfluidics in Comsol multiphysics?

16 October 2023 4,299 1 View

How can I determine the Length and width of the DLD Microfluidic?

I`m designing an Integrated microfluidic containing two DLDs, how can I determine the Length and width of the DLD Microfluidic? Are there any equations to calculate the Length and width of the DLD...

09 October 2023 4,968 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View