Is it possible to introduce a 2D axis-symmetric model in COMSOL Multiphysics to solve electromagnetic waves?

Hello dear researchers. I want to solve a cylindrical waveguide, lick an ordinary standard optical fiber. I am familiar enough with the 2D-axis-symmetric model, but as I knew from the equations...

11 January 2021 6,663 6 View

How can I design a beam splitter?

I would like to make a beam splitter by a glass for a specific angle. First I calculated the thickness of the partially reflecting layer by using of the transfer matrix then I coated this layer by...

25 September 2020 7,283 1 View

Effect of anesthetics drugs on coronavirus patients?

What is the reason for the ineffective or insignificant effect of Neuromuscular relaxants such as Atracurium and anesthetics such as Ketamine on patients with Coronavirus hospitalized in the...

22 September 2020 5,734 4 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

What kind of amides does the tendon consist of?

In visible reflectance spectroscopy performed on a bovine tendon, peaks were observed corresponding to tendon amides. But I do not know these peaks are related to what kind of amides.

16 August 2020 4,411 3 View

How to calculate the Reconnaissance Drought Index (RDI) in DrinC software?

30 April 2020 906 2 View

Where should I register my systematic review and meta-analysis?

Dear colleagues, Is it alright to register a systematic review and meta-analysis (focusing on human health studies) with the International Platform of Registered Systematic Review and...

25 March 2020 5,382 9 View

What are the crystals formed in the phosphate buffer solution at alkaline condition?

There are some crystals formed on the surface of the glass-ceramic substrate (mainly composed of Al, Si, Ca, O) after being treated with PBS (pH> ~10). I know that buffer salt crystalization is...

16 March 2020 4,385 3 View

How can I find data set for fake news?

Hi, Everyone. I am a PhD student and I need some data about fake news from twitter or any other platform that has available data. Can you please let me know how I can find data?

26 January 2020 6,415 12 View

Has any one experience with woun healing assay in a549 cell line?

I am going to study migration in a549 cell line after a treatment but the a549 cells which I am working with show no migration into the space created by a yellow tip even in normal condition (with...

13 August 2019 1,089 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View