I have ligation problem after ligation and transformation in Bl21 cells ,I do miniprep and restriction digestion i find un expected bands ?

Vector pET expression vector 5738 bp Insert : 3700 bp transformation :Bl21 chemically competent cells . Restriction enzymes : Not1 , Pst1 Ligation molar ratio : 1:1 after ligation &...

03 April 2020 1,805 0 View