I want to automate the measuring of neurite outgrowth in PC12 cells. Is there any programm or fiji plugin that can measure cellbody length ,neurite length and number of neurites in a unstained cells ?
Unfortunately, we are not aware of any existing program or plugin for automated quantification of PC12 differentiation. However, manual quantification can be done using basic image analysis software, such as ImageJ. With these tools, you can analyze images of PC12 cells to count them and calculate important features such as cell area, circularity, or shape. However, this is a time consuming process. It would be interesting to see if any specialized software or plugin for automated quantification of PC12 differentiation can be developed in the future.
I tested this program but i couldn´t get it to work https://www.biorxiv.org/content/10.1101/2023.04.27.538564v1
I don't think there is a plugin that can be used, at least there is no plugin that does it well. During my thesis I was looking for something that would help me quantify but finally we decided that the most efficient thing is to carry out morphological measurements by hand. We measured parameters such as
- Population differentiation percentage
- Average number of neurites per cell
- Average neurite length
- Average main neurite length
- Neurite length distribution
- Polarity index.
It's hard work but not particularly difficult. You just need patience.
I leave you a link to the document in case you want to take a look.
http://hdl.handle.net/10347/18669
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