How do you start building a data set for machine learning?

-Required are DEM/DTM satellite data from mountain ranges without glaciers. For example SRTM 30m. -The data has 3 parameters only. X, Y and Elevation. There is no need for georeference or...

22 January 2021 4,059 6 View

Where can I find dynamic real-world graph datasets?

For an experimental evaluation, I am looking for dynamic real-world graph datasets. In contrast to a traditional (static) graph, in a dynamic graph edges can be inserted and deleted, so the...

14 October 2020 1,618 3 View

Ion torrent - What happens if the amplicons are too long?

Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...

11 August 2020 368 3 View

Unable to add authors?

I am not able to add Authors where I normally can put their names.

17 March 2020 8,404 1 View

How can I coat silica nano/micro particles with a thick gold layer?

18 December 2019 5,660 3 View

Is it conceivable to investigate geospatial data with LiDAR as a freelancer?

This is a monetary question: There are products like the Phoenix LiDAR Systems. Attached to a drone it is possible to mine highly precise geospatial data very fastly. Would universities around the...

01 August 2019 1,538 2 View

qPCR primer efficiency question?

22 May 2019 4,117 4 View

When looking at SNQ on MRI, you select circular areas of the image which gives you a figure - what exactly is being measured here?

Signal-to-noise quotient (SNQ) is often used to describe the appearances of an MRI but it is not clear to me exactly what the image viewer is measuring, I assume that the sequence that you are...

06 April 2019 271 2 View

Which ICC for idea evaluation relialibility?

18 November 2018 1,647 2 View

Is there a Software to depict multi-channel linguistic data transcripts?

Related to my PhD-research I am looking for software that is capable of displaying multi-channel transcripts of linguistic data. More precisely, I do have video data, that includes writte (chat)...

25 February 2018 5,263 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

How to do immunostaining with 3D culture (tissue embedded in matrigel)?

I dissected tissue from mice then embedded it in matrigel for culture. For analysis, I plan to do immunostaining with some cell markers. But the regular IF protocol did not work. Matrigel would...

11 January 2021 6,961 3 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View

How do you fix organoids for IHC staining and H&E sections??

I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...

29 November 2020 1,900 1 View

Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View