I´m currently working with the THP-1 cellline.
I have two questions.
1. I got those cells as a gift from a nother group which told me to cultere this cellline in RPMI + Glutamin + Pen/Strep.
i recently read that they also need 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate an beta-mercaptoethanol.
Are those compounds necessary?
2. Is there a protocol for neutrophil differentiation?
I found a protocol using dibutryl cAMP would this work ?
Hi,
yes THP1 cells need all these components
use RPMI 1640 medium (it includes everything)+10% Fbs+penStrep.
differentiation PMA 50ng/ml for 48 hours.
To your 1st question,
THP-1 cells need all the mentioned. Fortunately, RPMI 1640 Medium comes with almost all that needed but require supplementing with 10% FBS and Pen/Strep.
To your 2nd question,
THP-1 cells can be differentiate into neutrophil-like cells by treatment with 0.5 mM dibutyryl cAMP for 3 days.
Vinod Nadella I'm interested in neutrophil differentiation from THP-1 too. Could you link some references, please? Thank you.
@ Valentino.. Here are few for your ref...
https://www.jimmunol.org/content/jimmunol/171/8/4227.full.pdf
https://www.karger.com/Article/FullText/495970
Article Honokiol suppresses formyl peptide-induced human neutrophil ...
https://www.jimmunol.org/content/190/12/6511.long
https://pubmed.ncbi.nlm.nih.gov/16630555/
Article Dipeptide HCH6-1 inhibits neutrophil activation and protects...
- Badges
- Science method