I would like to ask about a suitable protocol for synthesis of chitosan/siRNA complex and should it include poloxamer 188 as a stabilizer or not. Can I use poloxamer 407 instead?
thanks a lot for the replies. sorry my reply was repeated by mistake. my work will include both in vitro and in vivo parts. can i use ionic gelation method to synthesize chitosan/siRNA without poloxamer 188? if so. do i use poloxamer for the in vivo part only. so how to stabilize the particles for in vitro work?
Not sure what method you are using to synthesis chitosan/siRNA complext. Poloxomer 407 is a good stabilizer. You can go through some articles (Int J Biol Macromol, 67(2014): 124-131; Eur J Pharm Biopharm 93(2015): 149-164) that might help you.