Dear fellows,
I am facing a problem using Isotype control,
for example, when I run flow , the signal in un-stimulated samples is 10%, and in stimulated samples is 12%. However, the isotype control is 30% . isotype control should be negative, right ?
I am using the same concentration and the same staining period for isotype control and the target antibodies,
Can someone help me to understand why I am facing this problem.
Thank you in advance!
Doaa Glal
Hi christopher,
Thank you for your response, I got your point , and I will try FMO technique.
However, I face this problem while doing intracellular staining since my target is intracellular. Have you faced this problem with your intracellular staining before, and how you overcome it ?
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