Doaa Abdelmonsif

10 Questions 10 Answers 0 Followers

Questions related from Doaa Abdelmonsif

How to calculate miRNA relative expression in stem cell-derived exosomes?

I am currently working on stem cell-derived exosomes and need to assess exosomal miRNA relative expression by RT-PCR. can i use the stem cells of origin as the calibrator for calculation of fold...

13 August 2018 3,525 3 View

Does tissue homogenization for hydroyproline colorimetric assay need protease inhibitor?

dear colleagues i am using hydroxyproline colorimetric assay kit from biovision. can i homogenize my tissue samples in dH2O without protease inhibitor?

11 May 2016 8,699 1 View

What is the best protein to assess central IR after 6 weeks of STZ induced sporadic Alzheimer’s Disease in rat model?

i am working on STZ induced sporadic Alzheimer’s Disease model. i was wondering about the best protein to assess by western blot after 6 weeks of induction considering that i have limited...

17 January 2016 6,212 4 View

Is there a need to use poloxamer 188 for preparation of chitosan/siRNA?

I would like to ask about a suitable protocol for synthesis of chitosan/siRNA complex and should it include poloxamer 188 as a stabilizer or not. Can I use poloxamer 407 instead?

11 May 2015 9,208 6 View

Is DMBA induced breast cancer or Ehrlich tumor a better animal model for ER positive breast cancer?

dear all, I would like to work on tamoxifen in breast cancer. which model to use for ER positive tumor: DMBA induced breast cancer or Ehrlich tumor. I am afraid in case of DMBA that the rats...

27 March 2015 7,631 1 View

How can I inject substance P via the IV route?

i would like to inject substance P intravenously in rats. i will use Substance P acetate salt hydrate from sigma-Aldrich. is it better to dissolve in water or saline. is it better to use...

27 December 2014 7,454 5 View

Could you tell me about the most rapidly growing HER2 positive and triple negative cell line for xenograft breast cancer models?

Does it need E2 supplements and matrigel? How long would it take for the tumor to appear?

23 February 2014 1,020 3 View

Can anyone help with the xenograft breast cancer model?

I want to inoculate MCF7 or T47D cell lines after female albino mice irradiation. I read that it requires E2 pellet and that cell lines should be injected with Matrigel. Could you send me your...

02 February 2014 9,043 8 View

ATR1RII A1166-C polymorphism.

I bought a primer pair for conventional PCR, the sequence and Tm of both are: - GCA CCA TGT TTT GAG GTT G Tm 50.7 C - CGA CTA CTG CTT AGC ATA Tm 40.3 C By checking the Tm...

14 February 2013 280 3 View

I want to check for the possible molecular target of a new drug but I don't have access to scifinder database.

Is there any free database that could help and/or other alternative?

06 November 2012 1,570 0 View