8 Questions 10 Answers 0 Followers
Questions related from Doaa Abdelmonsif
I am currently working on stem cell-derived exosomes and need to assess exosomal miRNA relative expression by RT-PCR. can i use the stem cells of origin as the calibrator for calculation of fold...
13 August 2018 3,507 3 View
i am working on STZ induced sporadic Alzheimer’s Disease model. i was wondering about the best protein to assess by western blot after 6 weeks of induction considering that i have limited...
17 January 2016 6,181 4 View
I would like to ask about a suitable protocol for synthesis of chitosan/siRNA complex and should it include poloxamer 188 as a stabilizer or not. Can I use poloxamer 407 instead?
11 May 2015 9,191 6 View
dear all, I would like to work on tamoxifen in breast cancer. which model to use for ER positive tumor: DMBA induced breast cancer or Ehrlich tumor. I am afraid in case of DMBA that the rats...
27 March 2015 7,610 1 View
i would like to inject substance P intravenously in rats. i will use Substance P acetate salt hydrate from sigma-Aldrich. is it better to dissolve in water or saline. is it better to use...
27 December 2014 7,439 5 View
Does it need E2 supplements and matrigel? How long would it take for the tumor to appear?
23 February 2014 998 3 View
I want to inoculate MCF7 or T47D cell lines after female albino mice irradiation. I read that it requires E2 pellet and that cell lines should be injected with Matrigel. Could you send me your...
02 February 2014 9,018 8 View
I bought a primer pair for conventional PCR, the sequence and Tm of both are: - GCA CCA TGT TTT GAG GTT G Tm 50.7 C - CGA CTA CTG CTT AGC ATA Tm 40.3 C By checking the Tm...
14 February 2013 264 3 View
