10 October 2018 1 3K Report

I would like to convert acetate peak area in HPLC to concentration. Standard values of HPLC are available.

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What software is used for data analysis of HPLC outputs?

The area values (for HPLC) are on excel sheet. I would like to know which software I should use to analyse the data. Thanks

09 October 2018 2,490 1 View

Challenging with Standard Curve Points in qPCR assay!

The copy number of my samples in qPCR do not stand between standard curve points, out of them located. Does it give us correct right yield? * I have at least four s. curve points.

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Every Other One Standard Curve In Absolute qPCR

I am going to run a qPCR assay for the absolute module. I am not sure about something actually. Based on the common protocols, there should be 5 dilution points of standard curve, but I have it...

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Why is a plasmid used in the standard curve of qPCR assay?

I am going to use a plasmid in the standard curve of qPCR assay according to a protocol. I got curious to know why it is. I looked it up but could not find a clear answer. Thanks a zillion

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Does gDNA co-extract with Plasmid, and vice versa?

For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...

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Is there a formula to convert a peak area' value of HPLC to concentration?

Conversion of peak area' values of HPLC to concentration!

01 January 1970 6,053 6 View

Please introduce a reliable source regarding universal 16S rRNA

The universal 16S rRNA gene and universal 16S rRNA primers are admittedly used to identify both bacteria and archaea domains at the same time. I would like to learn more about its feature, and...

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Overlapping in forward and reverse reads

I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...

01 January 1970 7,904 6 View

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