What software is used for data analysis of HPLC outputs?

The area values (for HPLC) are on excel sheet. I would like to know which software I should use to analyse the data. Thanks

09 October 2018 2,463 1 View

Every Other One Standard Curve In Absolute qPCR

I am going to run a qPCR assay for the absolute module. I am not sure about something actually. Based on the common protocols, there should be 5 dilution points of standard curve, but I have it...

01 January 1970 1,979 0 View

Challenging with Standard Curve Points in qPCR assay!

The copy number of my samples in qPCR do not stand between standard curve points, out of them located. Does it give us correct right yield? * I have at least four s. curve points.

01 January 1970 3,506 0 View

Why is a plasmid used in the standard curve of qPCR assay?

I am going to use a plasmid in the standard curve of qPCR assay according to a protocol. I got curious to know why it is. I looked it up but could not find a clear answer. Thanks a zillion

01 January 1970 9,378 6 View

Does gDNA co-extract with Plasmid, and vice versa?

For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...

01 January 1970 1,684 3 View

Is there a formula to convert a peak area' value of HPLC to concentration?

Conversion of peak area' values of HPLC to concentration!

01 January 1970 6,023 6 View

Overlapping in forward and reverse reads

I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...

01 January 1970 7,868 6 View

Please introduce a reliable source regarding universal 16S rRNA

The universal 16S rRNA gene and universal 16S rRNA primers are admittedly used to identify both bacteria and archaea domains at the same time. I would like to learn more about its feature, and...

01 January 1970 3,599 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View