The copy number of my samples in qPCR do not stand between standard curve points, out of them located. Does it give us correct right yield?
* I have at least four s. curve points.
The area values (for HPLC) are on excel sheet. I would like to know which software I should use to analyse the data. Thanks
09 October 2018 2,490 1 View
I would like to convert acetate peak area in HPLC to concentration. Standard values of HPLC are available.
09 October 2018 3,067 1 View
I am going to run a qPCR assay for the absolute module. I am not sure about something actually. Based on the common protocols, there should be 5 dilution points of standard curve, but I have it...
01 January 1970 2,013 0 View
I am going to use a plasmid in the standard curve of qPCR assay according to a protocol. I got curious to know why it is. I looked it up but could not find a clear answer. Thanks a zillion
01 January 1970 9,400 6 View
For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...
01 January 1970 1,711 3 View
Conversion of peak area' values of HPLC to concentration!
01 January 1970 6,053 6 View
The universal 16S rRNA gene and universal 16S rRNA primers are admittedly used to identify both bacteria and archaea domains at the same time. I would like to learn more about its feature, and...
01 January 1970 3,626 2 View
I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...
01 January 1970 7,904 6 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...
30 July 2024 3,229 2 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,824 0 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View