The copy number of my samples in qPCR do not stand between standard curve points, out of them located. Does it give us correct right yield?
* I have at least four s. curve points.
The area values (for HPLC) are on excel sheet. I would like to know which software I should use to analyse the data. Thanks
09 October 2018 2,463 1 View
I would like to convert acetate peak area in HPLC to concentration. Standard values of HPLC are available.
09 October 2018 3,041 1 View
I am going to run a qPCR assay for the absolute module. I am not sure about something actually. Based on the common protocols, there should be 5 dilution points of standard curve, but I have it...
01 January 1970 1,979 0 View
I am going to use a plasmid in the standard curve of qPCR assay according to a protocol. I got curious to know why it is. I looked it up but could not find a clear answer. Thanks a zillion
01 January 1970 9,378 6 View
For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...
01 January 1970 1,684 3 View
Conversion of peak area' values of HPLC to concentration!
01 January 1970 6,023 6 View
I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...
01 January 1970 7,868 6 View
The universal 16S rRNA gene and universal 16S rRNA primers are admittedly used to identify both bacteria and archaea domains at the same time. I would like to learn more about its feature, and...
01 January 1970 3,599 2 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...
01 March 2021 2,327 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...
25 February 2021 2,615 3 View
I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...
24 February 2021 3,476 4 View
Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...
23 February 2021 9,159 3 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View