9 Questions 8 Answers 0 Followers
Questions related from Mehrdad Rabiei
The area values (for HPLC) are on excel sheet. I would like to know which software I should use to analyse the data. Thanks
10 October 2018 2,477 1 View
I would like to convert acetate peak area in HPLC to concentration. Standard values of HPLC are available.
10 October 2018 3,052 1 View
The universal 16S rRNA gene and universal 16S rRNA primers are admittedly used to identify both bacteria and archaea domains at the same time. I would like to learn more about its feature, and...
01 January 1970 3,614 2 View
I am going to run a qPCR assay for the absolute module. I am not sure about something actually. Based on the common protocols, there should be 5 dilution points of standard curve, but I have it...
01 January 1970 1,993 0 View
For the first time, I am going to extract plasmid. I would like to know whether gDNA would be extracted during the plasmid extracting? Similarly, is the plasmid extracted when gDNA getting to be...
01 January 1970 1,697 3 View
The copy number of my samples in qPCR do not stand between standard curve points, out of them located. Does it give us correct right yield? * I have at least four s. curve points.
01 January 1970 3,516 0 View
I have two forward and reverse reads from HiSeq Illumina platform. The PCR amplicon was 480 bp, 2*240. In merging, how will they have overlapping while they have no common bases? I am just...
01 January 1970 7,893 6 View
I am going to use a plasmid in the standard curve of qPCR assay according to a protocol. I got curious to know why it is. I looked it up but could not find a clear answer. Thanks a zillion
01 January 1970 9,388 6 View
Conversion of peak area' values of HPLC to concentration!
01 January 1970 6,039 6 View
