How to discriminate between active secretion and passive release due to cell death in vitro?

I am about to measure the secretion of a protein into the medium in a cell culture experiment (via exocytosis or extracellular vesicles). However, we have a certain level of death (10-20%) in our...

04 May 2018 3,104 4 View

How is it possible for a hydrophobic sensitive fluorescent probe to show different changes in intensity and blue shifting?

For a fluorescent probe which is sensitive to hydrophobic environment, what factors impact on the intensity and what factors impact the wavelength shifting? In our probe, we observed increase in...

06 July 2017 6,272 13 View

Drawing error-bar in excel is only based on standard deviation or should we divide it by the square root of iterations?

What do you think, is the drawing error-bar in excel only based on standard deviation or should we divide it by the square root of iterations?

10 November 2013 8,702 4 View

Can someone help in regards to a problem with low efficiency of competent cell preparation?

I am using the CaCl2 method for competent cell preparation but recently I am facing low efficiency of transformation in new competent cells despite several tries. I always test them by doing...

05 June 2013 9,735 17 View

What is the reason for blocking the development of pathology after fibrils injection in a-syn knock out mice?

It seems that endocytosis is the most important factor for expansion of a-syn in brain tissue. Additionally, it seems that knock-out mice have no problem in their essential functions of the brain,...

05 June 2013 1,783 3 View

Can anyone help me to find a protocol for gel extraction without using kits?

I used some kits for gel extraction but I did not get a satisfying result, so I want to do it without using a kit.

04 May 2013 8,838 22 View

How NaOH induce DNA fragmentation in cells, after just 15 min?

We have a DNA fragmentation ELISA kit from Roche. The protocol suggested NaOH treatment for 30 min as a positive control for DNA fragmentation. We did not get significant result, so I become...

04 May 2013 5,315 6 View

How can I treat cells with proteins without using common filters for sterilization?

I have to treat my cells with a-synuclein protein monomers, oligomers and fibrils but for sterilizing we have used 0.22 syringe filter for monomers before fibrillation induction. Now we found that...

04 May 2013 9,778 15 View

Requirement for a software to predict structural changes after a mutation.

I am inducing some mutations in a protein and I want to predict it's structural change. I need a software to figure out this problem. Any suggestions?

03 April 2013 8,307 6 View

Is it a reliable approach to stain the digestion product by EtBr for gel extraction before ligation?

In my cloning project, I have to double digest a plasmid and then extract the big fragment from a agarose gel to use it for ligation process. But for finding the place of digestion product on the...

02 March 2013 3,782 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View