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Questions related from Amir Tayaranian Marvian
I am about to measure the secretion of a protein into the medium in a cell culture experiment (via exocytosis or extracellular vesicles). However, we have a certain level of death (10-20%) in our...
05 May 2018 3,213 4 View
For a fluorescent probe which is sensitive to hydrophobic environment, what factors impact on the intensity and what factors impact the wavelength shifting? In our probe, we observed increase in...
07 July 2017 6,361 13 View
What do you think, is the drawing error-bar in excel only based on standard deviation or should we divide it by the square root of iterations?
11 November 2013 8,806 4 View
I need a protocol for isolating cytoplasmic content from cell membrane for cell culture cell line.
11 November 2013 2,466 8 View
It seems that endocytosis is the most important factor for expansion of a-syn in brain tissue. Additionally, it seems that knock-out mice have no problem in their essential functions of the brain,...
06 June 2013 1,873 3 View
I am using the CaCl2 method for competent cell preparation but recently I am facing low efficiency of transformation in new competent cells despite several tries. I always test them by doing...
06 June 2013 9,830 17 View
I used some kits for gel extraction but I did not get a satisfying result, so I want to do it without using a kit.
05 May 2013 8,920 22 View
I have to treat my cells with a-synuclein protein monomers, oligomers and fibrils but for sterilizing we have used 0.22 syringe filter for monomers before fibrillation induction. Now we found that...
05 May 2013 9,863 15 View
We have a DNA fragmentation ELISA kit from Roche. The protocol suggested NaOH treatment for 30 min as a positive control for DNA fragmentation. We did not get significant result, so I become...
05 May 2013 5,434 6 View
I am inducing some mutations in a protein and I want to predict it's structural change. I need a software to figure out this problem. Any suggestions?
04 April 2013 8,391 6 View
In my cloning project I am digesting a plasmid then keeping the digested fragments in -20C after gel extraction. As the ligation of these double digested fragments (not blunt end fragments) have...
03 March 2013 5,503 5 View
In my cloning project, I have to double digest a plasmid and then extract the big fragment from a agarose gel to use it for ligation process. But for finding the place of digestion product on the...
03 March 2013 3,867 11 View
In fact, I want to measure intracellular Ca++ in a cell line but because of some financial problems we need a cheaper indicator than common fluorescent indicators.
03 March 2013 3,072 3 View
I have a problem distinguishing my cells. The cells are treated, then stained with the Annexin-PI kit, and after that tested by flow cytometry. In the kit's manual, it is mentioned that these...
02 February 2013 6,140 59 View
Nowadays, famous companies like Merck and Thermo provide different ladders for native-PAGE analysis. However, basic biochemistry tells us that the movement of proteins on the native-PAGE is not...
01 January 1970 9,421 6 View
