3-(N-Morpholino)propanesulfonic acid,chemical formula is C7H15NO4S, Mwt 209.8,10 x MOPS Buffer is intended for use at 1x as the electrophoresis and gel running buffer during the separation of RNA on denaturing formaldehyde/agarose gels.10x MOPS Buffer is composed of 200 mM MOPS, pH 7.0, 80 mM Sodium Acetate, and 10 mM EDTA, pH 8.0 in Molecular Biology Grade Water.10x MOPS Buffer is tested for the absence of detectable RNase by incubation with yeast RNA for 14 to 16 hours at 37 °C. It is tested for the absence of detectable DNase by incubation with λ Hind III fragments (Exonucleases) and purified plasmid DNA (Endonucleases) for 14 to 16 hours at 37 °C. It also is tested for the absence of detectable protease by incubation with acetylated bovine serum albumin for 14 to 16 hours at 37 °C. After incubation, the samples are analyzed by electrophoresis.
MOPSO is β-Hydroxy-4-morpholinepropanesulfonic acid, 3-Morpholino-2-hydroxypropanesulfonic acid.MOPSO is a zwitterionic aminosulfonate buffer that is
very similar in structure to MOPS (3-morpholinopropanesulfonic acid), differing by the presence of a hydroxyl group on C-2 of the propane moiety. MOPSO falls into the class of Good buffers, which Good et al. developed to provide buffers in the
pH range of 6.15 - 8.35 for wide applicability to biochemical studies.The useful pH range for MOPSO is 6.5 - 7.9. MOPSO has been utilized as a buffer component in
the analysis of copper by two distinct methods: (1) a flow injection micellar echnique of the catalytic reaction of the reaction between 3-methyl-2-benzothiazolinone hydrazone with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline;2
(2) an electrospray-ionization quadrapole time-of-flight mass spectroscopy technique to measure the formation of chelating complexes of MOPSO with
copper.The effect of MOPSO and other Good buffers on the resolution of DNA using discontinuous electrophoresis on rehydratable polyacrylamide gels
has been examined.
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