Carcinogen for in vitro cancer induction?

More Mohammad Al-Deeb's questions See All

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Is it good choice to use the SARC/J auxiliary basis set with def2-SVPD basis set without including any relativistic effects like ZORA or whatever?

I’m currently utilizing the ORCA software for my computations, applying the PBE0 functional combined with the def2-SVPD basis set and incorporating the RIJCOSX approximation. For the auxiliary...

07 June 2024 6,352 0 View

Colony PCR Not Working?

I have inserted a 300bp sequence of interest into the PTRV2 vector for use in VIGS and transformed that vector into E.coli, plated on LB Agar w/ 1% Kanamycin. There was growth on the plates, but...

31 October 2023 5,559 4 View

How efficient is the removal of excess dye using a desalting column?

In protein labeling for conducting microscale thermophoresis how efficient is the removal of excess dye using a desalting column?

13 January 2021 4,138 4 View

If all else is perfect except for discriminant validity, the AVE is less than the MSV - is there any justification?

If all else is perfect and CFA and everything is good, except for discriminant validity, the AVE is less than the MSV - is there any article that could justify this?

27 December 2020 1,068 3 View

Ansys Fluent: How can I start drawing XY PLOT from a specific point?

Hello! # Ansys #Fluent: How can I start drawing XY PLOT "Nu VS curve length" from a specific point? (For example, in the case of two adjacent tubes, I want to compare the number of Nusselt...

05 November 2019 6,229 4 View

How to clean PTFE vessel from carbon deposits after it has been used for generating carbon from glucose?

A PTFE vessel was used as a reaction vessel to carbon coat magnetite NPs using glucose at 180C for 12 hrs. The interior of the vessel was blackened by the carbon deposits. It was soaked for long...

09 October 2019 5,766 3 View

What is the most popular English translation of the Quran? and Why?

There are many English translations of the Quran. In your opinion, which one is the best and why?

08 September 2019 3,950 3 View

How do you deal with slow learners? What action do you take if you feel that they will be unable to pass your course?

I believe that there are individual differences among students. I appreciate sharing your feedback in terms of how we can deal with very slow learners (slow comprehension).

08 September 2019 2,140 13 View

What is the marketing chaos and how can it occur in the business environment?

ماهي الفوضى التسويقية وكيف يمكن ان تحدث في بيئة الاعمال ؟

04 May 2019 5,570 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Adam B Shapiro

In vitro chemical transformation of cultured cells is a well-described assay for carcinogenicity. Here are 2 detailed articles on the subject.

http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?doclanguage=en&cote=env/jm/mono(2007)18

Mohammad Al-Deeb

Dear Adam

Thank you so much for your prompt response.

Best regards

Mohammad