I'm planning on doing a 16S rRNA library preparation using the Illumina MiSeq, and am following the recommended workflow by Illumina. Once the gDNA has been isolated using the Meta-G-Nome kit, it's recommended that the DNA should be resuspended in TE buffer. However, for the PCR amplification step of the desired 16S regions, Illumina asks that the DNA be in Tris buffer at pH 8.5. If I simply use the TE buffer provided with the DNA isolation kit and proceed to the PCR reaction, will that have any adverse reactions, or should I simply resuspend in the 10 mM, pH 8.5 Tris buffer?