Andrew Moore

4 Questions 2 Answers 0 Followers

Questions related from Andrew Moore

How to identify unknown species sequence data from 16S sequencing?

I recently used Illumina BaseSpace's 16S Metagenomics App to gather some preliminary data. After the analysis, there was identification of a number of "unknown species". I was wondering if there...

12 October 2016 4,747 3 View

Has anyone had experience importing samples into Illumina BaseSpace?

I'm trying to import FASTQ.gz files from a MiSeq 16S amplicon run into Illumina BaseSpace in order to use the 16S Metagenomics app. I have four samples, lets call them A-D. I uploaded all 4...

14 September 2016 8,453 1 View

Is there a difference between using Tris or TE buffer for PCR?

I'm planning on doing a 16S rRNA library preparation using the Illumina MiSeq, and am following the recommended workflow by Illumina. Once the gDNA has been isolated using the Meta-G-Nome kit,...

10 August 2016 4,328 1 View

Has anyone worked with C. elegans Habitation and Reproduction media (CeHR) and encountered constant contamination?

I've been working on using an axenic media, CeHR, to culture large numbers of C. elegans, but even though through working in a sterile biosafety cabinet I constantly am experiencing bacterial...

11 January 2016 7,287 2 View