Dear Amira,
For determination of protein yield, you should determine the total protein content of your target protein which is extracted from the bacterial biomass or harvested supernatant by a quantitative methods like ELISA. This amount is your protein yield.
After some purification steps like chromatography methods, you should determine the protein content by quantification methods like ELISA, HPLC, ....
By dividing this amount to first initial protein content (your target protein), you can calculate percent recovery.
Hi Amira, percent yield and percent recovery after chromatography steps are really the same thing if you are just measuring protein concentrations.
If you are measuring enzyme activities after chromatography, then it becomes important to measure enzyme activities divided by the total protein. This is specific activity and is important to determine how pure your enzyme is.
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