Atefeh Ebrahimian

4 Questions 5 Answers 0 Followers

Questions related from Atefeh Ebrahimian

For T cell culture, I sould use human serum AB off the clot, or usage of human serum AB is applicable?

There is any significance impact on T cell expansion to use human serum derived plasma or whole blood (off the clot)

06 June 2018 6,769 0 View

In viral vector production, In-process Test after harvest must be done per each lot? or it is approved to done for at least 3 batches?

According to pharmacopoeia 8, section 5.14 and 5.2.3; In viral vector production, some tests must be done as in-process tests after cell harvest for control cells (cells at or beyond the doubling...

04 April 2018 2,629 0 View

Can anyone help me to find a protocol for examination of extranous agent in cell culture and working cell bank?

According to pharmacopei 8, section 2.6.16 (atteched); a 14 day-incubation human cells by supernatant of cell culture is considered for evaluation of non-evidency of extraneous agents. I cannot...

03 March 2018 9,276 1 View

Does anybody know HPLC method for residual host cell RNA (E.coli) in produced DNA plasmid?

HPLC method; Residual E.coli Host Cell RNA

02 February 2018 2,389 4 View