Does anyone knows a free online software for determining the protein concentration from SDS-PAGE gels?

SDS-PAGE Protein concentration Online software

10 November 2018 1,796 0 View

Does anyone knows human tumor necrosis factor alpha quantification assays other than ELISA?

Human tumor necrosis factor alpha ELISA Assay Protein

10 November 2018 3,231 0 View

Is there a difference between percent yield and percent recovery calculation in case of protein purification?

Protein purification percent yield percent recovery

09 October 2018 9,444 3 View

Hi, I have a problem in Separating my peptide using 16% SDS-PAGE. Can any one help me to figure out the solution to this problem?

I work on highly positive charge short hydrophobic peptide production from an E. coli cell lysate, M.wt. 6.5 KDa. I used 16% SDS-PAGE with 0.376 M tris HCl as separating gel and 0.025 M tris HCl...

07 August 2018 7,178 5 View

What is the role of equilibration buffer in affinity chromatography?

Affinity chromatography Protein purification

01 February 2018 2,278 7 View

What is the difference between lysis of bacterial cells using sonication and lysis by boiling with SDS-PAGE sample buffer?

Bacterial cells lysis sonication SDS-PAGE

31 December 2017 4,207 9 View

Why will the primer not anneal to the DNA template if the annealing temperature is high?

In PCR, why will the primer not anneal to the DNA template if the annealing temperature is high?

31 December 2015 9,073 0 View

I wanted to know if i am designing a primer why its length should be between 18-30 nucleotide?. What happens if primer length exceed 30 nucleotide?

no details

11 December 2015 5,242 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View