Human tumor necrosis factor alpha
ELISA
Assay
Protein
SDS-PAGE Protein concentration Online software
10 November 2018 1,842 0 View
Protein purification percent yield percent recovery
09 October 2018 9,509 3 View
I work on highly positive charge short hydrophobic peptide production from an E. coli cell lysate, M.wt. 6.5 KDa. I used 16% SDS-PAGE with 0.376 M tris HCl as separating gel and 0.025 M tris HCl...
07 August 2018 7,216 5 View
Affinity chromatography Protein purification
01 February 2018 2,322 7 View
Protein Purification column chromatography
01 February 2018 2,694 2 View
Bacterial cells lysis sonication SDS-PAGE
31 December 2017 4,263 9 View
In PCR, why will the primer not anneal to the DNA template if the annealing temperature is high?
31 December 2015 9,126 0 View
no details
11 December 2015 5,280 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
