Does anyone knows a free online software for determining the protein concentration from SDS-PAGE gels?

SDS-PAGE Protein concentration Online software

10 November 2018 1,796 0 View

Does anyone knows human tumor necrosis factor alpha quantification assays other than ELISA?

Human tumor necrosis factor alpha ELISA Assay Protein

10 November 2018 3,231 0 View

Is there a difference between percent yield and percent recovery calculation in case of protein purification?

Protein purification percent yield percent recovery

09 October 2018 9,444 3 View

Hi, I have a problem in Separating my peptide using 16% SDS-PAGE. Can any one help me to figure out the solution to this problem?

I work on highly positive charge short hydrophobic peptide production from an E. coli cell lysate, M.wt. 6.5 KDa. I used 16% SDS-PAGE with 0.376 M tris HCl as separating gel and 0.025 M tris HCl...

07 August 2018 7,178 5 View

What is the role of equilibration buffer in affinity chromatography?

Affinity chromatography Protein purification

01 February 2018 2,278 7 View

I was wondering if there is a difference between equilibration and binding buffer ?

Protein Purification column chromatography

01 February 2018 2,642 2 View

What is the difference between lysis of bacterial cells using sonication and lysis by boiling with SDS-PAGE sample buffer?

Bacterial cells lysis sonication SDS-PAGE

31 December 2017 4,207 9 View

I wanted to know if i am designing a primer why its length should be between 18-30 nucleotide?. What happens if primer length exceed 30 nucleotide?

no details

11 December 2015 5,242 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View