Amira hassan sabry

9 Questions 9 Answers 0 Followers

Questions related from Amira hassan sabry

Does anyone knows a free online software for determining the protein concentration from SDS-PAGE gels?

SDS-PAGE Protein concentration Online software

11 November 2018 1,825 0 View

Does anyone knows human tumor necrosis factor alpha quantification assays other than ELISA?

Human tumor necrosis factor alpha ELISA Assay Protein

11 November 2018 3,260 0 View

Is there a difference between percent yield and percent recovery calculation in case of protein purification?

Protein purification percent yield percent recovery

10 October 2018 9,493 3 View

Hi, I have a problem in Separating my peptide using 16% SDS-PAGE. Can any one help me to figure out the solution to this problem?

I work on highly positive charge short hydrophobic peptide production from an E. coli cell lysate, M.wt. 6.5 KDa. I used 16% SDS-PAGE with 0.376 M tris HCl as separating gel and 0.025 M tris HCl...

08 August 2018 7,205 5 View

What is the role of equilibration buffer in affinity chromatography?

Affinity chromatography Protein purification

02 February 2018 2,309 7 View

I was wondering if there is a difference between equilibration and binding buffer ?

Protein Purification column chromatography

02 February 2018 2,674 2 View

What is the difference between lysis of bacterial cells using sonication and lysis by boiling with SDS-PAGE sample buffer?

Bacterial cells lysis sonication SDS-PAGE

01 January 2018 4,244 9 View

Why will the primer not anneal to the DNA template if the annealing temperature is high?

In PCR, why will the primer not anneal to the DNA template if the annealing temperature is high?

01 January 2016 9,109 0 View

I wanted to know if i am designing a primer why its length should be between 18-30 nucleotide?. What happens if primer length exceed 30 nucleotide?

no details

12 December 2015 5,272 4 View