Is fold change true representative of differential gene expression?

Hi Everyone, I have a simple query. When we estimate differential gene expression between two data sets or two conditions, we generally observed log(base 2)fold change. However, the fold change...

09 October 2018 2,694 4 View

Regarding fluorescence microscopy ?

Hi Guys, I am trying to make a stable cell line from NIH/3T3 by transfecting with my plasmid of interest. After transfection, while I was changing the medium, I applied a bit harsh pressure on...

09 October 2018 1,489 3 View

What are locus specific primers? How are they different from normal primers?

Hi everyone! I cam across term called "locus specific primer". I came across this term in 3C (chromatin conformation capture) method. I tried to google about it but could not understand much. Are...

06 July 2018 8,459 1 View

Do anyone know about an offline tool for RNA seq analysis?

Hello everyone I have to travel a lot in next few days but I don't want to waste time. Does anyone know any tool which can be used to perform RNA seq data analysis offline (from fastq files). I...

06 July 2018 4,462 4 View

Why it is important to take equal amount of total RNA for reverse transcription reaction (cDNA preparation)?

Hey everyone, I am working on liver tissue samples. I have FACS sorted 3 populations from same tissue and I want to study the gene expression between these populations using qPCR. One of the...

05 June 2018 6,359 11 View

What are the two population clouds in FACS data?

Dear all, I am trying to determine the DNA content in cell population by Hoechst 33342 DNA specific stain using flow cytometry analysis (BD FACS fortessa). I am observing two different clouds (...

04 May 2018 6,325 4 View

Karyotyping protocol for AML-12 cell lines?

Hi everyone, I was wondering if anyone have done the karyotyping for AML-12 cell lines (adult mouse liver cell lines)? I believe the protocol for karyotyping should be same like any other adherent...

31 December 2017 424 1 View

Regarding fluorescence microscopy ?

Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...

10 November 2017 9,315 2 View

Culturing conditions for HepG2?

09 October 2017 4,234 5 View

Is anyone using formaldehyde to fix cells and extract RNA from these cells later?

07 August 2017 6,346 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View