Is fold change true representative of differential gene expression?

Hi Everyone, I have a simple query. When we estimate differential gene expression between two data sets or two conditions, we generally observed log(base 2)fold change. However, the fold change...

09 October 2018 2,694 4 View

Regarding fluorescence microscopy ?

Hi Guys, I am trying to make a stable cell line from NIH/3T3 by transfecting with my plasmid of interest. After transfection, while I was changing the medium, I applied a bit harsh pressure on...

09 October 2018 1,489 3 View

What are locus specific primers? How are they different from normal primers?

Hi everyone! I cam across term called "locus specific primer". I came across this term in 3C (chromatin conformation capture) method. I tried to google about it but could not understand much. Are...

06 July 2018 8,459 1 View

Why it is important to take equal amount of total RNA for reverse transcription reaction (cDNA preparation)?

Hey everyone, I am working on liver tissue samples. I have FACS sorted 3 populations from same tissue and I want to study the gene expression between these populations using qPCR. One of the...

05 June 2018 6,359 11 View

What are the two population clouds in FACS data?

Dear all, I am trying to determine the DNA content in cell population by Hoechst 33342 DNA specific stain using flow cytometry analysis (BD FACS fortessa). I am observing two different clouds (...

04 May 2018 6,325 4 View

Karyotyping protocol for AML-12 cell lines?

Hi everyone, I was wondering if anyone have done the karyotyping for AML-12 cell lines (adult mouse liver cell lines)? I believe the protocol for karyotyping should be same like any other adherent...

31 December 2017 424 1 View

Regarding fluorescence microscopy ?

Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...

10 November 2017 9,315 2 View

Culturing conditions for HepG2?

09 October 2017 4,234 5 View

Is anyone using formaldehyde to fix cells and extract RNA from these cells later?

07 August 2017 6,346 2 View

Use of proteinase k to degrade RNAse?

07 August 2017 3,974 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View