17 Questions 17 Answers 0 Followers
Questions related from Ankur B. Sharma
Hi Everyone, I have a simple query. When we estimate differential gene expression between two data sets or two conditions, we generally observed log(base 2)fold change. However, the fold change...
10 October 2018 2,756 4 View
Hi Guys, I am trying to make a stable cell line from NIH/3T3 by transfecting with my plasmid of interest. After transfection, while I was changing the medium, I applied a bit harsh pressure on...
10 October 2018 1,537 3 View
Hi everyone! I cam across term called "locus specific primer". I came across this term in 3C (chromatin conformation capture) method. I tried to google about it but could not understand much. Are...
07 July 2018 8,598 1 View
Hello everyone I have to travel a lot in next few days but I don't want to waste time. Does anyone know any tool which can be used to perform RNA seq data analysis offline (from fastq files). I...
07 July 2018 4,516 4 View
Hey everyone, I am working on liver tissue samples. I have FACS sorted 3 populations from same tissue and I want to study the gene expression between these populations using qPCR. One of the...
06 June 2018 6,410 11 View
Dear all, I am trying to determine the DNA content in cell population by Hoechst 33342 DNA specific stain using flow cytometry analysis (BD FACS fortessa). I am observing two different clouds (...
05 May 2018 6,382 4 View
Hi everyone, I was wondering if anyone have done the karyotyping for AML-12 cell lines (adult mouse liver cell lines)? I believe the protocol for karyotyping should be same like any other adherent...
01 January 2018 474 1 View
Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...
11 November 2017 9,363 2 View
Hello All, I just started using HepG2 cell lines and I would like to know the culturing conditions. I have read through the ATCC guidelines for this cell line and they recommend EMEM+10% FBS....
10 October 2017 4,289 5 View
I want to sort cells using FACS, followed by fixation of sorted populations using formaldehyde or ethanol. I would like to extract total RNA from these sorted and fixed cells later. Has anyone...
08 August 2017 6,426 2 View
Hello Everyone! I am trying to extract intact and high integrity RNA from fixed cells. The idea is to sort cells using FACS and from sorted population extract RNA. The samples is mouse tissue...
08 August 2017 4,025 6 View
Hi, I am trying to find few genes are expressed mouse cell lines. I found few suggestions like oncomine but it does not include mouse cell lines. For example, I am trying to find whether Cd45 is...
06 June 2017 6,128 3 View
Hey Guys! I am cloning my transgene in a vector. I observed that it have SV40_3_splice site and SV40_int site . I was wondering what are the functions of these site? Please give a reference of any...
11 November 2016 6,249 0 View
Hello All, I am using AML 12 cells (liver cell lines - non cancerous ) . I observed a change in their ploidy over late passages. Is it observed by anyone else also or it might be due to...
10 October 2016 9,848 9 View
I am interested to test the efficacy of my compound on pancreatic cancer cell lines. NCCS pune dont have them. Can someone share their freeze or help me to get one. ( except ATCC Obviously )
05 May 2016 9,670 2 View
Hey everyone ! I am working on Y chromosome based study and planning to use BNL CL.2 mouse embryonic cell lines. I am wondering what was the gender of embryonic mouse from which BNL CL.2 cells...
01 January 1970 4,527 2 View
Hey guys, I wanted to sort Binucleated and tetraploid cells (from tissue or cell lines )using some techniques like FACS (rather than microscopic techniques ). Can you guys suggest...
01 January 1970 6,472 16 View
