Can anyone provide a detailed Congo red staining protocol for detecting amyloid aggregates in neuroblastoma cell culture please?

I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...

31 December 2018 3,512 4 View

Holding my PhD degree before post-doc is a must?

I am going to finish my PhD research on December but I'm not allowed to defend my thesis until September for some special reason. Can I do a post-doc in that 6 months? if not what do you suggest...

05 June 2018 4,083 3 View

Do you have a detailed protocol for dissolving Abeta25-35 in DMSO?

Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...

08 September 2017 9,563 2 View

For peptide concentration determination, measured molar extinction coefficient or calculated one?

07 August 2017 2,786 4 View

Does the choice of solvent affect the molar extinction coefficient of a particular peptide?

Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...

06 July 2017 8,887 1 View

For transferring cell lines between laboratories in 2 hours distance should I keep the T-flask vertical or horizontal during communication?

01 February 2017 1,940 7 View

What kit do you recommend for trans-membrane peptide and protein extraction?

I'm looking for a kit to extract a trans-membrane peptide, preferably, with no or very low contamination to cytosolic proteins.

01 January 1970 925 2 View

What is the best protein assay selection for a peptide as small as 1000 Da?

Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...

01 January 1970 7,192 9 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View