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I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...
31 December 2018 3,615 4 View
I am going to finish my PhD research on December but I'm not allowed to defend my thesis until September for some special reason. Can I do a post-doc in that 6 months? if not what do you suggest...
05 June 2018 4,375 3 View
Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...
08 September 2017 9,670 2 View
Hey, There are some calculation you can predict the amount of epsilon (molar extinction coefficient) by amino acid content. You may also prepare a solution of the protein of interest with known...
07 August 2017 2,939 4 View
Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...
06 July 2017 8,989 1 View
The cell line is SH-SY5Y neuroblastoma.
01 February 2017 2,069 7 View
I'm looking for a kit to extract a trans-membrane peptide, preferably, with no or very low contamination to cytosolic proteins.
01 January 1970 1,027 2 View
Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...
01 January 1970 7,295 9 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
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05 August 2024 4,407 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco
29 July 2024 2,163 1 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
