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Questions related from Mona Abdollahi
I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...
01 January 2019 3,590 4 View
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08 August 2018 6,353 0 View
I am going to finish my PhD research on December but I'm not allowed to defend my thesis until September for some special reason. Can I do a post-doc in that 6 months? if not what do you suggest...
06 June 2018 4,192 3 View
Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...
09 September 2017 9,645 2 View
Hey, There are some calculation you can predict the amount of epsilon (molar extinction coefficient) by amino acid content. You may also prepare a solution of the protein of interest with known...
08 August 2017 2,901 4 View
Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...
07 July 2017 8,966 1 View
The cell line is SH-SY5Y neuroblastoma.
02 February 2017 2,041 7 View
I'm looking for a kit to extract a trans-membrane peptide, preferably, with no or very low contamination to cytosolic proteins.
01 January 1970 1,004 2 View
Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...
01 January 1970 7,269 9 View