Mona Abdollahi

9 Questions 19 Answers 0 Followers

Questions related from Mona Abdollahi

Can anyone provide a detailed Congo red staining protocol for detecting amyloid aggregates in neuroblastoma cell culture please?

I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...

01 January 2019 3,585 4 View

Is there a database that I can use to look into solubility and solvents of a chemical?

08 August 2018 6,347 0 View

Holding my PhD degree before post-doc is a must?

I am going to finish my PhD research on December but I'm not allowed to defend my thesis until September for some special reason. Can I do a post-doc in that 6 months? if not what do you suggest...

06 June 2018 4,186 3 View

Do you have a detailed protocol for dissolving Abeta25-35 in DMSO?

Hello, I have no access to HFIP, and I already tried NH4OH method with little success. The ThT assay resaults did not make sense by this method. And AFM showed the 500 nm spheres were formed. Any...

09 September 2017 9,640 2 View

For peptide concentration determination, measured molar extinction coefficient or calculated one?

Hey, There are some calculation you can predict the amount of epsilon (molar extinction coefficient) by amino acid content. You may also prepare a solution of the protein of interest with known...

08 August 2017 2,893 4 View

Does the choice of solvent affect the molar extinction coefficient of a particular peptide?

Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...

07 July 2017 8,960 1 View

For transferring cell lines between laboratories in 2 hours distance should I keep the T-flask vertical or horizontal during communication?

The cell line is SH-SY5Y neuroblastoma.

02 February 2017 2,035 7 View

What kit do you recommend for trans-membrane peptide and protein extraction?

I'm looking for a kit to extract a trans-membrane peptide, preferably, with no or very low contamination to cytosolic proteins.

01 January 1970 997 2 View

What is the best protein assay selection for a peptide as small as 1000 Da?

Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...

01 January 1970 7,263 9 View