I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells.
Thank you in advanced
Asking only for one additional parameter: your cells in culture after probably staining positive you expect to be "dead"?
If the latter is true: why not try a Congo Red staining kit (if that is available) or - at least - to read and learn from the following article:
Research article Open Access
Endogenous amyloidogenesis in long-term rat hippocampal cell cultures
Sarah J Bertrand, Marina V Aksenova, Micheal Y Aksenov, Charles F Mactutus and Rosemarie M Booze
BMC Neuroscience 2011 12:38
https://doi.org/10.1186/1471-2202-12-38
© Bertrand et al; licensee BioMed Central Ltd. 2011
Received: 20 December 2010 Accepted: 10 May 2011 Published: 10 May 2011
"...
B. Live cells stained with Congo red dye. Staining with Congo Red is a classical method of the detection of amyloids. ... C. Congo red staining can also be observed in what appears to be the inside of cells, as well as the network.
cf.: Staining of non-fixed hippocampal cells with Congo Red and microscopic imaging of the results was carried out as previously described [39].
[39]: · Aksenov MY, Aksenova MV, Mactutus CF, Booze RM: HIV-1 protein-mediated amyloidogenesis in rat hippocampal cell culture. Neuroscience Letters. 2010, 45: 174-178. View Article (available only by PPV @
https://www.sciencedirect.com/science/article/abs/pii/S0304394010003940?via%3Dihub) Google Scholar
If you - what we do not know for now - process the mentioned neuroblastoma cells from culture to embeddings (frozen or cryo-sections or Paraffin-sections) and have access to ResearchGate you might enter the following URL (when you already are in ResearchGate website):
https://www.researchgate.net/post/Does_anybody_have_a_detailed_Congo_Red_staining_protocol_for_mouse_brain_sections?
where you'll find some Staining recipes... too
e.g. [001] Jakov Milić Re = now 2 year ago [=Mar 10, 2017]
&&
Best of luck,
W.H.M
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