Can anyone provide a detailed Congo red staining protocol for detecting amyloid aggregates in neuroblastoma cell culture please?

I plan to seed neuroblastoma cells. Differentiate them with retinoic acid, treat them with AB1-42 and then stain them with Congo red to observe amyloid aggregates in those cells. Thank you in...

31 December 2018 3,512 4 View

Is there a database that I can use to look into solubility and solvents of a chemical?

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07 August 2018 6,274 0 View

Holding my PhD degree before post-doc is a must?

I am going to finish my PhD research on December but I'm not allowed to defend my thesis until September for some special reason. Can I do a post-doc in that 6 months? if not what do you suggest...

05 June 2018 4,083 3 View

For peptide concentration determination, measured molar extinction coefficient or calculated one?

07 August 2017 2,786 4 View

Does the choice of solvent affect the molar extinction coefficient of a particular peptide?

Hi everybody, I am trying to determine peptide concentration by far-UV spectroscopy and I was wondering if different solvents (water, ammonium chloride,Ham's F-12 medium and PBS) that I use as...

06 July 2017 8,887 1 View

For transferring cell lines between laboratories in 2 hours distance should I keep the T-flask vertical or horizontal during communication?

01 February 2017 1,940 7 View

What kit do you recommend for trans-membrane peptide and protein extraction?

I'm looking for a kit to extract a trans-membrane peptide, preferably, with no or very low contamination to cytosolic proteins.

01 January 1970 925 2 View

What is the best protein assay selection for a peptide as small as 1000 Da?

Which method is the best choice to determine protein concentration of amyloid beta peptide (25-35) with a molecular weight of 1060.28 g/mol? Is Bradford assay incompatible because it responds to...

01 January 1970 7,192 9 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View