How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

BSEM/SEM approach?

Hi all! I'm getting into BSEM approach and I am specially concerned about when it is recommended or not to use this model. I have tested a BSEM with a small sample obtaining excellent results,...

07 September 2020 3,919 3 View

Unable to perfiorm raster to-table join in ArcGIS?

Dear all, I am trying to replicate some results from a publication. There, one of the datasets they use is WISE30sec map (available for download here:...

18 February 2020 6,906 3 View

Low 260/230 ratio using Qiagen DNeasy kit?

Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...

16 February 2020 787 3 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How data is normalized in Transcriptome Analysis Console (TAC) Software?

I'm using TAC software 4.0.2 (Affimetrix) for the first time to analize differential gene expression in two groups of samples (control and treatment). I have problems with the normalization step...

18 November 2019 7,146 3 View

Importance of drying-time after using gelatin as coating?

I am culturing stem cells on plates coated with gelatin. After finding so many variations of drying time in different protocols, I am wondering what is the reason? In other words, why is it...

09 September 2019 9,415 3 View

How to interpret ordinal logistic regression in Stata with ordinal dependant and independent variables?

My question is very straightforward. I have different ordinal independent variables (4 categories), and I am trying to regress it on different ordinal and nominal variables. I want help on how I...

09 July 2019 5,769 1 View

Could you suggest any scale to assess parents' satisfaction with their children's schools?

Hi all! I'a looking for a scale to assess parents' satisfaction with their children's schools. I have found some different papers about parents satisfaction but no one seems to offer just what I...

10 December 2018 8,430 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

No title

02 March 2021 3,060 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View