Is there a database on internet of specific primers for bacteria identification?

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How Artificial Intelligence (AI) can change the way to write a thesis?

How Artificial Intelligence can change the way to write academic thesis under master and Pd.D. levels are affecting the originality and knowledge of future academics.

09 June 2024 1,156 9 View

Qiagen QIAamp DNA Stool Kit or QIAamp PowerFecal DNA Kit?

Hello colleagues, I want to determine which kit is better for extracting DNA from insects in bird feces, with the purpose of conducting barcoding later on. So far, we have been using a modified...

07 February 2024 2,601 1 View

Is there any research in the caribean about the legal framework for artificial intelligence for the protecction of fundamental rights?

I am making a research on artificial intelligence and fundamental rights. I would like to have some information about the topic in countries in the caribbean. Please, let me know if you have some...

17 January 2024 1,345 0 View

Fluorescence properties changed due to laser irradiation?

Hi everybody. I would like to know if thre is any material whose fluorescent properties can be changed, in a permanent way, by a external stimulus, in particular by laser irradiation. For...

16 January 2024 8,821 4 View

How to estimate "output-specific" returns to scale of a multiproduct industry using DEA?

I am estimating a DEA for an industry with a multiproduct technology (two inputs and two ouptuts). When I run the "complete" model I find ambiguous results regarding scale economies. When I run...

06 January 2024 5,063 2 View

How to compare two wavefronts if Piston is different?

I have two wavefronts, one where Zernike 00, has a value 0 and the other with a value -0.07 for the same Zernike. How I should check the rest of the Zernike to compare the aberrations in both...

22 November 2023 5,515 0 View

Have anyone taken one of this courses? Is this company reliable?

Hi all, does anyone of you now this website that it is offering courses? Any feedback will be highly appreciated :) Nano Science & Technology...

28 August 2023 9,091 3 View

How can I dissolve alpha tocopherol?

Hello, I need to dissolve alpha tocopherol, I used DMSO and ethanol but it didn't have the effect I expected to take it to uv visible spectroscopy

27 August 2023 1,251 2 View

QPCR related problem?

I have a situation which quite do not understand. We are trying to get a qPCR to work. We extracted RNA and synthersized cDNA from have 5 Wts samples, 3 iRNA samples, and 1 genomic WT control. We...

24 August 2023 2,050 2 View

How to produce a thin layer with perpendicular magnetic anisotropy on Al substrate or between two Al layers??

Hello everybodoy. I am interested in any reference about thin magnetic monolayeres with perpendicular magnetic anisotropy produced ( in particular by PVD ) on Al substrate or sandwiched between...

17 July 2023 912 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View