Is there a better reagent for extracting RNA/miRNA from tissue than TRIZOLE?

More Nauman Rauf Khan's questions See All

For how long we can put a plasma in a room temperature before sifting it to -80C for storage?

Plasma is required for miRNA analysis but in our settings we have to take sample from long distant place sometime from outskirts so that the sample(plasma) seperated cant be stored straight away...

10 November 2019 9,341 3 View

I want to extract micro RNA from oral tissues. How is liquid nitrogen used and cryovials should contain which solution for better transportation?

Should I use rna later solution in cryovial after the snap freeze

31 December 2018 6,489 3 View

Should cancer patients assessment for miRNA levels exclude patients with systemic disease?

Cancer group patient assessment for miRNA levels shall exclude patients with systemic disease? I am working on HNSCC and if the sample i am obtaining are from patients who also have systemic...

03 April 2018 2,354 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Prof. Gheita

Dear Nauman Rauf Khan

Check this previously presented on RG

https://www.researchgate.net/post/What_is_the_best_way_for_extracting_total_miRNA_from_human_plasma

Arnaud Beurdeley

Dear Nauman,

I would recommend either Qiagen miRNeasy Serum/plasma advanced kit or the Norgen one. We are currently use them on either serum and plasma.

Best.

Arnaud

Fahmida Khan Ema

Hi Nauman

For miRNA/RNA isolation from serum/plasma/biofluid you can use glycogen along with trizol for better extraction, and for better purification you can use QIAGEN kit. Good luck